FastQCFastQC Report
Mon 6 Feb 2017
SRR2078240_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078240_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2241269
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30250.034.875771
GGTATCA15150.032.087381
TCAACGC37050.027.2651064
ATCAACG37750.027.0317023
TATCAAC39000.026.868272
CAACGCA37800.026.7235345
AACGCAG39400.025.6325876
CGATACG700.001338420624.62963341
ACGCAGA47550.021.0222037
GTACATG64750.020.0205421
CGCAGAG51500.019.4106888
TACATGG65400.019.2900832
CGTGATC4750.018.88167256
AGAGTAC49700.018.6642711
ACATGGG67000.018.5750983
CAGAGTA54700.017.21037910
GTATAGA4450.017.0447431
GCGATAC3054.7293724E-1116.95924614
GCAGAGT58700.016.3900859
GCACCGT5100.016.220426
Produced by FastQC (version 0.11.5)