FastQCFastQC Report
Mon 6 Feb 2017
SRR2078240_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078240_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2241269
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17450.041.1355251
GTATCAA40250.039.975531
TATCAAC58300.027.5295242
TCAACGC58000.027.2498844
ATCAACG59050.027.0588173
CAACGCA59050.026.8240345
AACGCAG61650.025.6365496
ACGCAGA73300.021.5849137
GCGATAC3400.021.30869314
CGCAGAG76450.020.3492138
AGAGTAC73900.020.21480611
GTACATG58000.019.550681
GAGTACT48200.018.96772612
TGCGATA3850.018.81806813
GTGCGAT4050.018.74062512
CAGAGTA81050.018.60178210
TACATGG61150.018.3101652
GCAGAGT84650.018.2154059
AGTACTT51450.017.5013513
GTACTTT51450.017.3672414
Produced by FastQC (version 0.11.5)