FastQCFastQC Report
Mon 6 Feb 2017
SRR2078239_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078239_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2072456
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29500.035.300861
GGTATCA14150.033.1423151
ATCAACG36200.028.9507923
TCAACGC36250.028.7213524
CAACGCA36800.028.1970485
TATCAAC36750.028.0464482
AACGCAG39100.026.7069916
GCGATAC3700.024.23279214
ACGTAAT1151.6986851E-624.00009356
ACGCAGA44150.022.950747
TGCGATA3800.022.68758413
CGATACT3800.022.68539215
ATAGGCG3200.022.64071356
TAGGCGC3300.021.95197757
CGCAGAG47850.021.0345618
GTGCGAT4300.020.04949412
AGAGTAC44050.020.04081711
GTACATG57500.019.9099711
TTCGGTG4550.019.71960627
TACATGG59450.019.1928792
Produced by FastQC (version 0.11.5)