FastQCFastQC Report
Mon 6 Feb 2017
SRR2078239_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078239_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2072456
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34250.039.6866041
GGTATCA15050.032.0255621
TATCAAC48400.027.6440962
ATCGCGA500.00775708727.58484663
CAACGCA48100.027.1646445
TCAACGC48300.027.1239194
ATCAACG48750.027.0868243
AACGCAG51600.025.4564176
TTCGACG750.00199937422.98848258
CGTGCCG3850.022.40056610
ACGCAGA59450.021.8396537
GTGCCGA4900.021.12053511
CGCAGAG61650.021.0364078
GCGATAC3300.020.9066914
GTACATG52600.020.6336981
TGCGATA3200.020.48202513
AGAGTAC60850.019.61531311
TACATGG55200.019.4034882
CGATACT4000.018.97190715
CAGAGTA65600.018.66827810
Produced by FastQC (version 0.11.5)