FastQCFastQC Report
Mon 6 Feb 2017
SRR2078238_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078238_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2307549
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30600.034.1320341
GGTATCA16100.032.5430831
TCGCGAG500.007760400427.58256138
TCAACGC37150.027.374824
CAACGCA37550.026.8060465
ATCAACG37900.026.7409843
TATCAAC38600.026.1627622
AACGCAG40450.025.0508616
ACGCAGA47550.020.7295827
GTATTAG4550.020.454711
GTACATG54350.019.724311
TACATGG55650.019.1380022
AGAGTAC47450.018.82481411
CGCAGAG52050.018.7411948
CGTGATC4000.018.11284656
CGTGCCG3051.8189894E-1218.09203110
ACATGGG58050.017.5775663
GAGTACT22250.017.36021612
GTATAAG7950.017.3433841
CAGAGTA53650.016.97076210
Produced by FastQC (version 0.11.5)