FastQCFastQC Report
Mon 6 Feb 2017
SRR2078238_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078238_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2307549
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37650.038.2161031
GGTATCA16700.037.1620641
TATCAAC51300.028.0358832
ATCAACG51900.027.7063373
TCAACGC52250.027.3881154
CAACGCA53050.027.0404155
GCGATAC2700.026.8305114
AACGCAG55750.026.0415946
ACGCAGA63750.022.4837577
CGCAGAG65900.021.5144278
GTGCGAT3950.020.9598212
GTACATG56550.020.170961
AGAGTAC67900.019.91586111
GAGTACT42100.019.91120712
TGCGATA3600.019.16464813
CAGAGTA71900.018.85586510
TACATGG58550.018.8227792
GTACTTT44550.018.81620214
AGTACTT45150.018.79536213
GCAGAGT74600.018.449279
Produced by FastQC (version 0.11.5)