FastQCFastQC Report
Mon 6 Feb 2017
SRR2078237_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078237_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1909303
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26500.033.9565241
GGTATCA14400.031.8432771
TACCGTA703.879657E-529.5408087
TCAACGC32750.026.6340874
TATCAAC33700.026.28562
CAACGCA33200.026.1678665
ATCAACG33400.026.114393
AACGCAG36300.024.3053866
GCACCGT4350.022.1839036
ACGCAGA40450.021.300547
GTACCGT1304.969019E-621.2087846
CGCAGAG42100.020.3046598
GGCACGC4600.019.51357746
AGAGTAC40300.019.42357611
CGCCCAA4450.019.40112150
GTACATG50800.019.2066381
TACATGG50100.018.8507022
CGTGCCG3750.018.39107310
TACCGAT3250.018.0274687
GTACCGA3650.017.9403086
Produced by FastQC (version 0.11.5)