FastQCFastQC Report
Mon 6 Feb 2017
SRR2078237_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078237_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1909303
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13950.041.0206341
GTATCAA33550.039.4878461
TATCAAC46450.028.0674692
CAACGCA47100.027.6735655
TCAACGC47100.027.6735654
ATCAACG47200.027.5414923
AACGCAG48950.026.5568626
TATCGTG1502.8974682E-822.99413111
ACGCAGA56500.022.5627567
CGTGCCG3550.022.34640910
GCACCGT4200.022.2856
GTATACG952.9600036E-421.903841
CGCAGAG59850.021.208738
GTGCCGA3800.020.87625111
CGTCGTA1004.3437228E-420.6947210
AGAGTAC58050.020.5580611
GTACATG46200.019.6676241
TACATGG46400.019.2050862
CAGAGTA63400.019.20408810
GAGTACT37800.018.52256612
Produced by FastQC (version 0.11.5)