FastQCFastQC Report
Mon 6 Feb 2017
SRR2078236_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078236_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2455251
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17400.034.880521
GTATCAA34500.033.9843831
TCAACGC44250.026.101324
CAACGCA44750.025.9616555
ATCAACG45250.025.8292663
TATCAAC45800.025.291712
AACGCAG47700.024.2783416
GTACATG70400.021.4056431
GTGGTAT10100.020.4856361
GCGATAC3050.020.35047114
TACATGG71900.020.0424942
ACGCAGA57750.019.5157417
TGCGATA3050.019.2198913
ACATGGG74100.018.9833533
CGCAGAG59400.018.6277758
AGAGTAC56550.018.5977411
GTATAGG4550.018.1894421
TGGTATC11250.018.0802292
CGTTAAG1200.001472890417.25791723
CAGAGTA62500.016.88240610
Produced by FastQC (version 0.11.5)