FastQCFastQC Report
Mon 6 Feb 2017
SRR2078236_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078236_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2455251
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46000.040.469961
GGTATCA19900.037.6285061
CGTGCCG3450.028.99964710
TCAACGC65550.028.2291724
TATCAAC65700.028.1693362
ATCAACG66150.027.9731273
CAACGCA66100.027.9418625
AACGCAG69800.026.5599966
ATCGACG658.666704E-426.53489915
GTGCCGA5250.022.99972211
ACGCAGA79450.022.9166167
CGCAGAG82450.021.968048
GAGTACT54650.021.65254412
GCACCGT5950.021.5483676
AGAGTAC82100.021.05272911
GTACATG63050.020.5087911
AGCACCG7000.020.2962325
TCGACGT850.004125189520.29097716
TACATGG64750.020.1255992
AGTACTT59800.020.0766813
Produced by FastQC (version 0.11.5)