FastQCFastQC Report
Mon 6 Feb 2017
SRR2078235_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078235_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2181566
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17000.037.1073231
GTATCAA33700.033.4483951
TCAACGC42500.025.7938164
ATCAACG43300.025.3957023
CAACGCA43000.025.3329665
CGTATAC851.4520601E-424.332673
TATCAAC45350.024.319822
AACGCAG45450.024.0388166
GTACATG66500.020.7346061
ACGCAGA53650.020.1076977
TACATGG67500.019.7603632
GTATACG1258.801709E-519.3039191
GTGGTAT9300.019.2742651
CGCAGAG55750.019.043358
ACATGGG69900.018.6411233
AGAGTAC53650.018.0614911
CAGAGTA58100.017.15294310
CATGGGG48550.016.9701654
GCAGAGT63800.016.2150219
TACCGTC1503.5537218E-416.0840157
Produced by FastQC (version 0.11.5)