FastQCFastQC Report
Mon 6 Feb 2017
SRR2078235_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078235_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2181566
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47600.042.4506721
GGTATCA18850.041.738561
TATCAAC64600.030.6236152
TCAACGC66100.030.1341724
ATCAACG66750.029.840733
CAACGCA67750.029.1957515
AACGCAG71100.027.8201476
CGCACGA2000.025.86061335
ACGCAGA83800.023.4077267
GAGTACT56950.023.20019712
TAGCGCG902.134902E-422.98773629
CGCAGAG86900.022.5494048
AGTACTT60950.021.84742413
GTACTTT60700.021.71007314
AGAGTAC86500.021.57584411
CAGAGTA91500.020.6607510
CGCGCGA850.00413190120.28515839
GCAGAGT94950.020.1611339
TACTTTT68150.019.33722115
GTACATG65250.019.2818661
Produced by FastQC (version 0.11.5)