FastQCFastQC Report
Mon 6 Feb 2017
SRR2078234_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078234_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1034855
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6100.038.4381681
GTATCAA15050.035.51231
ATCAACG19450.027.6559493
GCGTTAC500.007749562627.58904343
TCAACGC20050.026.829644
CAACGCA21150.025.5972865
TATCAAC21050.025.5488912
AACGCAG22850.023.6837236
GTACATG34850.023.0534931
GTGTATA4700.022.7429851
TACATGG35600.021.595062
ACATGGG35500.020.6887783
TGCGCAA850.004128230720.28704310
ACGCAGA27450.019.7139117
CGCAGAG27600.019.234918
TGTTCGA1108.1883516E-418.83441251
AGAGTAC27250.018.35139811
CATGGGA14100.018.0974434
CATGGGG24300.017.3124354
CAGAGTA29750.017.15704210
Produced by FastQC (version 0.11.5)