FastQCFastQC Report
Mon 6 Feb 2017
SRR2078234_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078234_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1034855
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13380.1292934758975895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22450.046.80731
GGTATCA6650.039.6350361
TACGAAC459.731071E-538.5209584
TATCAAC33150.031.2714732
ATCAACG34300.030.524773
TCAACGC34550.030.404244
CAACGCA34800.029.986575
AACGCAG36700.028.339676
ACGCAGA43050.024.0671067
CGCAGAG44100.023.5427688
ACGGTGT750.001998644622.98859441
GTACATG29900.022.501831
TACATGG29900.022.1474062
AGAGTAC44000.022.03054611
CAGAGTA46600.021.0974810
GCAGAGT48200.020.8940899
GTCTACG1004.1842792E-420.8083931
TGTGCGA1004.3375985E-420.69773710
GAGTACT30700.020.33816712
ACATGGG30500.020.1193073
Produced by FastQC (version 0.11.5)