FastQCFastQC Report
Mon 6 Feb 2017
SRR2078233_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078233_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2375022
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34450.032.9272461
GGTATCA18550.032.1551781
TCAACGC42300.026.3225844
CAACGCA42850.026.064625
ATCAACG43100.025.8334543
TATCAAC44500.025.0186082
AACGCAG45750.024.4800456
CGGCACG3350.021.63392822
ACGCAGA53050.020.6580417
CGTGCCG3350.020.58723410
CGCAGAG55550.019.5438678
GTACATG62250.019.2747461
AGAGTAC52700.019.04123911
TACATGG62250.018.5492382
GAGTACT26350.018.190612
GCACCGT4950.018.1003976
CAGAGTA58900.017.56381210
GCAGAGT61000.017.5244649
ACATGGG63700.017.4250353
ACCGTGC6850.017.1069418
Produced by FastQC (version 0.11.5)