FastQCFastQC Report
Mon 6 Feb 2017
SRR2078233_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078233_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2375022
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50050.040.2354351
GGTATCA19650.040.2169341
TATCAAC69250.028.7710722
ATCAACG70450.028.2300283
TCAACGC70350.028.220914
CAACGCA70500.028.0625695
AACGCAG73950.026.7533636
ACGCAGA87450.022.5113377
CGCAGAG90300.021.6969038
AGAGTAC88250.021.02965711
GAGTACT60000.020.58241812
GTACATG62750.020.4373531
CAGAGTA95500.019.6498910
TACATGG64200.019.3221742
GCAGAGT98050.019.3127279
GTACTTT64150.019.08957714
AGTACTT64550.019.07816513
CGTGCCG3700.018.64630910
ACATGGG66000.018.4265333
TACTTTT71650.017.1387915
Produced by FastQC (version 0.11.5)