FastQCFastQC Report
Mon 6 Feb 2017
SRR2078232_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078232_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7829
Sequences flagged as poor quality0
Sequence length75
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.1021841869970622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1200.046.01
TATCAAC1600.034.52
TCAACGC1800.030.6666664
ATCAACG1850.029.837843
CAACGCA1950.028.3076935
AACGCAG2000.027.66
ACGCAGA2250.024.5333357
CGCAGAG2150.024.0697698
AGAGTAC1901.4551915E-1123.60526311
CAGAGTA1952.1827873E-1123.010
GCAGAGT1952.1827873E-1123.09
AGTACTT1955.838956E-1021.2307713
GAGTACT2008.021743E-1020.712
ACTTTTT2502.7284841E-1119.3216
GTACTTT2151.9790605E-919.25581614
TACTTTT2202.6357156E-918.81818215
CTTTTTT3001.8189894E-1117.2517
Produced by FastQC (version 0.11.5)