FastQCFastQC Report
Mon 6 Feb 2017
SRR2078231_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078231_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2257042
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17700.036.2301141
GTATCAA36750.035.086841
TCAACGC48050.027.405784
ATCAACG48850.026.8140453
CAACGCA49000.026.6627965
AACGCAG50700.025.7607786
TATCAAC50900.025.3255312
CGCGTAA700.00133700624.63411110
GTACATG75950.022.606411
GTATACG953.0807164E-421.7749771
TACATGG75350.021.6362842
ACGCAGA61050.021.0552547
CGCAGAG62200.020.5588078
ACATGGG77750.020.5269243
AGAGTAC60950.018.67261711
TTGCGCG950.00781866918.16516118
CATGGGG51700.018.06974
CGCGATC1150.00110868618.00480523
CCTATAC4500.017.618083
GTGGTAT9600.017.5976581
Produced by FastQC (version 0.11.5)