FastQCFastQC Report
Mon 6 Feb 2017
SRR2078231_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078231_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2257042
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20500.039.950041
GTATCAA48150.039.0626831
TCAACGC64950.028.67434
ATCAACG65100.028.6082293
CAACGCA66300.028.2473665
TATCAAC65650.028.0031322
AACGCAG68850.027.2011646
ACGCAGA83150.022.1873577
TTACGCT952.9600016E-421.9040974
CGCAGAG85700.021.4516228
GAGTACT52150.021.36488212
GTACATG73600.020.981731
TACATGG73350.020.618252
AGAGTAC85350.020.41031811
AGTACTT56450.019.98186913
ACATGGG76500.019.3128283
GTACTTT56800.019.19070814
CAGAGTA91750.019.06179610
GCAGAGT93300.018.8148949
TATCGTA1501.6973416E-518.3971712
Produced by FastQC (version 0.11.5)