FastQCFastQC Report
Mon 6 Feb 2017
SRR2078230_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078230_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2371435
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29500.034.9540441
GGTATCA14800.031.6902371
CAACGCA37150.027.014175
TCAACGC37600.026.7831484
ATCAACG38050.026.6454243
TATCAAC38850.026.1821862
AACGCAG40400.025.0006436
ACGCAGA46150.021.5873957
GTACATG62950.021.5848391
TACATGG62800.021.2484022
CGCAGAG48150.020.4064988
ACATGGG65000.019.7360173
CGTGATC6150.019.6330656
GCGATAT900.00574992519.15872613
AGAGTAC46850.018.84384311
CCGTGAT6450.018.71989355
CGGTATG3350.018.53589659
CTCGACA3500.017.75237347
CCCGGTA4150.017.45465357
CCGGTAT3800.017.2461358
Produced by FastQC (version 0.11.5)