FastQCFastQC Report
Mon 6 Feb 2017
SRR2078230_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078230_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2371435
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36300.037.3410761
GGTATCA17450.034.170371
TATCAAC53250.025.2474252
CGTGCCG4400.025.08741210
CAACGCA54350.024.6674585
ATCAACG54800.024.5281123
TCAACGC55200.024.2876154
AACGCAG56650.023.6659566
GCGATAC3650.021.73669414
GTACATG62200.021.680831
TACATGG62450.021.2506082
GCACCGT6350.020.1855686
GTGCCGA5400.019.80279511
AGCACCG7800.019.5420915
TGCGATA3900.019.45882613
ACGCAGA67400.019.425427
ACATGGG65950.019.3306313
GGCACGC6800.018.76192346
ATCCCGG6450.018.71200660
CGCAGAG70450.018.5605168
Produced by FastQC (version 0.11.5)