FastQCFastQC Report
Mon 6 Feb 2017
SRR2078229_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078229_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1644636
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17550.034.190291
GGTATCA9200.030.3618221
GTATACG658.6806505E-426.5269471
TCAACGC22850.025.6523554
ATCAACG23250.025.3585533
CAACGCA23900.024.3811025
TATCAAC24050.024.3701782
AACGCAG24600.023.6794016
GTACATG42850.021.2463231
TACATGG42200.020.9147362
CGCCTAG850.00413493220.28222710
GCGATAC1409.394815E-619.70273414
ACGCAGA29300.019.293397
CGGCACG2406.0754246E-1018.69077722
CGCAGAG30850.018.439138
ACATGGG44750.018.3372923
TGCGATA1351.584551E-417.87840713
AGAGTAC30350.017.15468611
ACCGTAT1653.8666E-516.7154358
GTATTAC2904.2382453E-1016.6479471
Produced by FastQC (version 0.11.5)