FastQCFastQC Report
Mon 6 Feb 2017
SRR2078229_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078229_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1644636
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26700.037.514161
GGTATCA12300.036.0672871
GCGCGTT756.187813E-527.58510436
TATCAAC36400.027.2249532
ATCAACG36700.026.5270923
CAACGCA37000.026.3120085
TCAACGC37150.026.2990284
CGTGCCG1901.8189894E-1225.42356310
AACGCAG39000.024.9626756
GTACATG43300.022.0917341
TATACTG5200.021.3204335
TACATGG44750.021.13842
CGCGTTG1004.3517243E-420.68882837
ACGCAGA46900.020.4743397
GTACTAG2051.6461854E-920.287851
TCGTATG2402.910383E-1120.11474840
ACATGGG44600.020.0416873
CGCAGAG48600.019.7347168
TTCGACG1056.045213E-419.70424358
AGAGTAC47650.019.33349411
Produced by FastQC (version 0.11.5)