FastQCFastQC Report
Mon 6 Feb 2017
SRR2078228_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078228_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2473601
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32100.034.5968361
GGTATCA15950.034.165041
ATCAACG40900.026.471983
TATCAAC41400.026.3167132
TCAACGC41300.026.2161224
CAACGCA42300.025.5942825
AACGCAG44400.024.4539766
GTATACG851.4469714E-424.3454511
ACGCAGA52850.020.2167917
CGCAGAG54100.019.49768
GTACATG68100.019.3971181
TACATGG69250.018.421582
AGAGTAC52950.017.64893711
ACATGGG72300.016.9782053
TGCGATA3106.184564E-1116.68571513
CAGAGTA58900.016.45152910
GAGTACT21800.016.4509912
GCGATAC3157.8216544E-1116.42086214
TAGGACA10300.016.4039214
GCAGAGT62850.015.9662499
Produced by FastQC (version 0.11.5)