FastQCFastQC Report
Mon 6 Feb 2017
SRR2078228_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078228_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2473601
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45700.040.1880381
GGTATCA19350.037.9657551
TATCAAC62350.029.2267532
ATCAACG64050.028.6086433
TCAACGC64750.028.2458674
CAACGCA65200.027.8915375
AACGCAG69050.026.5370566
ACGCAGA81000.022.383697
CGCAGAG83200.021.6012888
AGAGTAC82200.020.81914911
GAGTACT52200.020.688412
GTACATG65450.019.8015481
CAGAGTA89000.019.42230810
GCAGAGT91400.019.2870719
AGTACTT57200.019.06093213
GTACTTT57700.018.77616514
TACATGG67150.018.6764282
CGCTCGA1150.001110131718.0014110
ACATGGG68300.017.8011133
CGTGCCG3950.017.46972310
Produced by FastQC (version 0.11.5)