FastQCFastQC Report
Mon 6 Feb 2017
SRR2078227_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078227_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2402272
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28150.033.4381451
GGTATCA15600.029.1747571
TCAACGC33400.027.8724564
ATCAACG34900.026.6728293
CAACGCA35250.026.308545
TATCAAC36250.025.3909952
AACGCAG37200.025.015366
ACGCAGA44750.020.2570327
GTACATG59200.019.6275521
CGCAGAG47000.019.2155468
TACATGG58250.018.76032
AGAGTAC45500.018.56730311
ACATGGG60250.017.9109273
CAGAGTA49500.017.41521810
GTACTAC4400.016.456011
GCGATAC2101.0039967E-616.42006314
GTGCGAT3900.015.9148312
GCAGAGT55050.015.8473759
CGCACGA3351.9099389E-1015.47686635
TACCGTT1350.003264677715.3190057
Produced by FastQC (version 0.11.5)