FastQCFastQC Report
Mon 6 Feb 2017
SRR2078227_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078227_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2402272
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39550.038.7530331
GGTATCA18450.037.0254021
TATCAAC56100.027.1889512
TCAACGC56200.026.8904654
ATCAACG56700.026.8367273
CAACGCA57700.026.1313325
AACGCAG60550.024.9586126
CGTGCCG3400.024.35182410
ACGCAGA71300.021.071127
GTACATG57750.020.2352141
CGCAGAG74050.020.2170878
GCACCGT5400.019.8983046
TACATGG58000.019.8432462
GAGTACT49400.019.62277612
AGAGTAC73950.018.94030811
GTGCCGA4650.018.54753711
ACATGGG58850.018.4940193
CAGAGTA78650.018.20323410
AGCACCG7200.017.8121915
AGTACTT54050.017.80695313
Produced by FastQC (version 0.11.5)