FastQCFastQC Report
Mon 6 Feb 2017
SRR2078226_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078226_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270813
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15700.036.459741
GGTATCA6950.031.7541391
TCAACGC19800.028.387534
CAACGCA20250.027.9258825
ATCAACG20550.027.3504093
AACGCAG21250.026.9277636
TATCAAC21300.026.8687882
ACTCGAC1052.2730288E-523.01044346
ACGCAGA25350.022.1646447
CTAGTAC1959.404175E-1021.2194793
CGCAGAG26950.020.596188
GTACATG32150.019.9497361
CGTTAAT1056.03546E-419.70846241
ATAACGC1056.0450827E-419.70383
AGAGTAC26200.019.34657711
GCGTTAA900.0057484619.1587440
TTAACGA1108.2471804E-418.81484853
TACATGG32850.018.7861272
GCACCGT2255.5933924E-918.3844176
CAGAGTA29550.017.97013310
Produced by FastQC (version 0.11.5)