FastQCFastQC Report
Mon 6 Feb 2017
SRR2078226_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078226_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270813
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24100.045.0173841
GGTATCA8650.038.0680351
TATCAAC32250.032.9881972
ATCAACG33350.032.100333
CAACGCA34350.031.1658235
TCAACGC34550.031.0856884
AACGCAG35700.029.793196
ACGCAGA41600.025.498157
CGCAGAG43400.024.4021388
AGAGTAC42600.023.48930511
GAGTACT29550.023.35270712
CAGAGTA46300.021.83576410
GCAGAGT47400.021.8342979
AGTACTT32150.021.78611413
GTACTTT32700.021.10313214
CTCGTAT1657.958806E-820.89894339
TACATGG30950.020.7138582
TATGCCG1851.1832526E-820.5035643
GTACATG31550.020.3247531
CCGTTAT1056.049607E-419.70161666
Produced by FastQC (version 0.11.5)