FastQCFastQC Report
Mon 6 Feb 2017
SRR2078225_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078225_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2452892
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14550.032.4648061
GTATCAA28500.032.3014221
TCAACGC35850.024.6164974
ATCAACG36300.024.5957283
TATCAAC36300.024.2143882
CAACGCA36300.024.1204155
AACGCAG37550.023.1263166
GTACATG60400.019.6370741
ACGCAGA45700.019.0028157
TACATGG60950.018.832612
CGCAGAG47550.017.8326428
ATAGGCG2551.369699E-917.58689156
ACATGGG63250.017.5493853
TCGATCG1250.001948292116.55129441
GCGATAC3551.8189894E-1216.51379214
AGAGTAC46500.016.31533211
TGCGATA3651.8189894E-1216.06135713
CGTCGTT1300.002515497915.92999746
TATACCG1300.00253684915.9098325
TCGCAAT2907.517883E-915.46876149
Produced by FastQC (version 0.11.5)