FastQCFastQC Report
Mon 6 Feb 2017
SRR2078225_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078225_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2452892
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40350.039.721331
GGTATCA17000.036.5282971
ATCAACG56300.028.6359523
TCAACGC56750.028.4088784
TATCAAC56750.028.3530182
CAACGCA57900.027.7847485
AACGCAG60150.026.8606996
CGTGCCG3750.025.75834810
ACGCAGA69550.023.2003027
GTGCCGA4650.022.25663811
CGCAGAG72600.022.1450168
GAGTACT46450.021.6855612
TATACCG1455.0518247E-721.5199645
AGAGTAC70600.021.40230811
GTACATG58000.020.6953811
CAGAGTA78300.019.69413810
AGTACTT51200.019.53896513
GCAGAGT82050.019.2989739
TACATGG59350.019.1646232
ACATGGG61200.018.9217853
Produced by FastQC (version 0.11.5)