FastQCFastQC Report
Mon 6 Feb 2017
SRR2078224_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078224_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2426485
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17850.034.960911
GTATCAA33350.032.978981
TCAACGC42600.025.4932634
ATCAACG43500.025.0435223
CAACGCA43100.024.9565125
TATCAAC44900.024.5667382
AACGCAG46000.023.6785536
GTACATG64650.020.1588461
ACGCAGA53650.020.0452187
GCGTTAG1751.4824218E-719.70416844
CGCAGAG55000.019.4929398
TACATGG67200.018.7226522
ACATGGG68250.018.3863833
GCACCGT5150.018.071016
AGAGTAC55350.017.94200711
GCGATAC2601.7844286E-917.24114614
GGACCGT2251.12055204E-716.8514046
CAGAGTA61250.016.72039410
GGTGCGA3309.094947E-1216.71868911
GCAGAGT65000.015.9149059
Produced by FastQC (version 0.11.5)