FastQCFastQC Report
Mon 6 Feb 2017
SRR2078224_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078224_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2426485
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18700.042.09541
GTATCAA42400.037.7856181
TCAACGC58650.026.9516434
TATCAAC58550.026.9429342
ATCAACG59550.026.6025263
CAACGCA59500.026.4500985
AACGCAG62100.025.5101496
ACGCAGA73300.021.4893077
GTACATG60550.020.7321721
CGCAGAG76750.020.3601538
CGGCACG3750.020.23271622
TACATGG62400.020.113352
AGAGTAC76000.019.33757411
GAGTACT49750.018.7920112
ACATGGG64800.018.2953013
CAGAGTA82650.017.906910
GCAGAGT85000.017.8162279
AGTACTT53050.017.75310513
GTATACG1200.001427437417.3388541
GTACTTT55750.016.8933114
Produced by FastQC (version 0.11.5)