FastQCFastQC Report
Mon 6 Feb 2017
SRR2078223_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078223_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2179751
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20600.036.653261
GTATCAA43650.035.0698931
ATCAACG56250.026.479933
TCAACGC56400.026.3495864
CAACGCA58650.025.338155
TATCAAC59600.025.2206332
AACGCAG62200.024.1032166
GTGGTAT10750.023.0919041
GTACATG84500.021.3801271
ACGCAGA71750.020.8489327
TACATGG86150.020.5294762
CGCAGAG72700.020.388728
ACATGGG87700.019.500143
TGGTATC11550.019.4020292
AGAGTAC71750.019.17593611
TATACCG1451.27203675E-519.0233785
GAGTACT34100.019.01117912
CGCAATA1301.1676317E-418.60584336
CAGAGTA75650.018.41526610
CGACGTT950.007755335418.1907735
Produced by FastQC (version 0.11.5)