FastQCFastQC Report
Mon 6 Feb 2017
SRR2078223_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078223_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2179751
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22160.1016629881119449No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24500.045.4221041
GTATCAA58600.040.3473551
TATCAAC83700.028.2822132
ATCAACG84900.027.9602413
TCAACGC85450.027.8208314
CAACGCA88500.026.9011945
AACGCAG92000.025.802446
CCGCTAG1301.8592254E-723.87094936
GTACATG82550.022.2580491
CGTTACG1253.5132634E-622.07445524
ACGCAGA109250.021.5650887
TACATGG85000.021.5294592
CGCGTAA1304.955544E-621.215765
CGCAGAG111900.021.0576698
ACATGGG87250.020.6536083
ATTGCGC1701.0926124E-720.28250530
GTGGTAT9100.020.191231
AGAGTAC111250.020.00502811
GAGTACT73800.019.82343912
TATTCCG1408.995974E-619.8024985
Produced by FastQC (version 0.11.5)