FastQCFastQC Report
Mon 6 Feb 2017
SRR2078222_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078222_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2263223
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27650.030.678161
GGTATCA16400.030.0664061
ATCAACG32650.024.1820763
GCGATAC2600.023.87884914
CAACGCA32850.023.6150265
TCAACGC33450.023.5011774
TATCAAC34900.023.4123482
TAGCGCG902.1310397E-422.9939379
AACGCAG34600.022.5142966
TGCGATA3050.021.48661213
GTGCGAT3450.020.9949312
GTACATG59200.020.2696761
TACATGG60400.018.893532
ACATGGG61200.018.7037163
ACGCAGA41100.018.7032857
GGTGCGA3750.017.47539311
ATTCGGT3950.017.46722826
CGCACAA2601.7898856E-917.23706425
GTATAGG5650.017.0883031
CGCAGAG45150.016.7235188
Produced by FastQC (version 0.11.5)