FastQCFastQC Report
Mon 6 Feb 2017
SRR2078222_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078222_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2263223
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17550.039.3097841
GTATCAA41000.038.1344381
TCAACGC56550.027.0196634
TATCAAC57200.026.898522
ATCAACG56900.026.853463
CAACGCA56850.026.755195
GCGATAC3200.025.87316714
AACGCAG59550.025.5421076
TCGCACG951.0539174E-525.41307422
TGCGATA3650.022.68332713
ATAGGCG2450.022.51906256
ACGCAGA70350.021.5043667
GTACATG57550.020.7825321
CGCAGAG73550.020.4963958
ATTCGGT4100.020.18878426
GCGCGTT1551.0103067E-620.02198828
TACATGG59500.019.8017432
AGAGTAC71150.019.58907911
CGTACAC2303.255991E-1019.5834623
GTGCGAT4700.019.08375512
Produced by FastQC (version 0.11.5)