FastQCFastQC Report
Mon 6 Feb 2017
SRR2078221_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078221_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1832465
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11900.039.686731
GTATCAA22950.031.9940031
TCAACGC29550.023.9168324
CAACGCA29900.023.520285
ATCAACG29900.023.5189953
TATCAAC31900.022.4736272
GTACATG51700.022.0703451
AACGCAG31800.021.9993616
TACATGG53350.020.9320162
GTGTAGC7800.019.0040231
ACATGGG56600.019.0019423
ACGCAGA38750.017.3431427
GTATAAT5700.016.9338171
CATGGGG42100.016.7053414
TAGCCCT7850.016.2494774
CGCAGAG41000.016.0595388
CGTGATC2402.3124267E-715.81152256
GTATAGG5050.015.7003071
GTACTAA3753.6379788E-1215.6274941
TATACTG6150.015.13464455
Produced by FastQC (version 0.11.5)