FastQCFastQC Report
Mon 6 Feb 2017
SRR2078221_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078221_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1832465
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13450.040.7329981
GTATCAA32750.039.7037541
TATCAAC46400.027.7148572
TCAACGC46250.027.5753694
CAACGCA46750.027.4287055
ATCAACG46950.027.2380493
AACGCAG48550.026.4831666
ACGCAGA56300.022.6356497
CGCAGAG59400.021.36998
AGAGTAC58200.020.51054611
GAGTACT35300.020.03509312
GTACATG48700.019.437721
CAGAGTA63550.019.21815910
CCCGGTA1801.9929757E-719.1574757
CGTCCGA1108.2386355E-418.81839811
CCGGTAT1651.8379833E-618.80915358
GCAGAGT66050.018.6971329
AGTACTT38800.018.49455313
TCAATCG1501.7034297E-518.38916445
TACATGG51800.018.134112
Produced by FastQC (version 0.11.5)