FastQCFastQC Report
Mon 6 Feb 2017
SRR2078220_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078220_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2450970
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30750.034.0924841
GGTATCA16700.031.8005331
CAACGCA36950.027.6174325
TCAACGC38000.027.0385274
ATCAACG39700.025.9664923
AACGCAG39600.025.9359976
TATCAAC41350.025.345172
CGTGCCG2650.023.42233510
GTACATG63350.021.992011
ACGCAGA47600.021.2154087
TACATGG66300.020.8510512
AGAGTAC47300.020.04818711
CGCAGAG50500.019.9321028
GCGATAC3300.019.85378314
ACATGGG69600.019.3191493
TCAATCG3450.018.98863445
TGCGATA3600.018.19929913
CAGAGTA54050.017.73587610
GTGCCGA3550.017.48427811
AACGTCG1402.0783568E-417.25589422
Produced by FastQC (version 0.11.5)