FastQCFastQC Report
Mon 6 Feb 2017
SRR2078220_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078220_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2450970
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20900.039.1588971
GTATCAA45750.038.279391
TATCAAC65050.026.964892
TCAACGC65150.026.8132364
ATCAACG65150.026.7068353
CAACGCA65400.026.657745
AACGCAG68150.025.6837646
ACGCAGA79500.021.7649027
GATCGCG800.002910455521.5495949
GTACATG63650.020.9217281
CGCAGAG82450.020.8353848
TACATGG66900.019.8457782
AGAGTAC80450.019.63902911
GAGTACT51850.019.42739912
GCACCGT5900.019.3863056
ACATGGG68450.019.0391483
AGTACTT54400.018.83380513
CAGAGTA86900.018.73711810
CGCACGA3500.018.71850435
GTGGTAT10300.018.5178361
Produced by FastQC (version 0.11.5)