FastQCFastQC Report
Mon 6 Feb 2017
SRR2078219_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078219_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1782271
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11650.034.9287031
GTATCAA25700.034.7530561
TCAACGC31750.026.7143444
ATCAACG32150.026.5949653
CAACGCA32600.025.9098575
TATCAAC33550.025.8933332
AACGCAG34150.024.8264436
CGCTTAT700.001339560724.62569438
CGTAGTA953.065825E-421.7902262
TAATTCG1605.730908E-821.55959959
TAAGGCG1605.765105E-821.54748341
ACGCAGA38950.021.2384437
CGTACAA1206.4350475E-520.10646425
CGCAGAG40950.020.0345028
GTACATG46600.019.3143881
AAGGCGT4000.018.9553936
CGTGCCG1651.8348182E-618.81247510
TACATGG47850.018.7314472
AGAGTAC41300.018.20513311
GAGTACT20450.018.04587212
Produced by FastQC (version 0.11.5)