FastQCFastQC Report
Mon 6 Feb 2017
SRR2078219_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078219_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1782271
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17600.043.9553761
GTATCAA42800.039.473441
CGTGCCG1850.031.70301210
ATCAACG57050.029.2331983
TCAACGC57200.029.1565364
TATCAAC57800.028.9171262
CAACGCA57700.028.7836995
AACGCAG59750.027.7961436
GTGCCGA2500.024.84024211
ACGCAGA69800.023.710357
TCAATCG1202.4739275E-622.9840945
CGCAGAG71700.022.8513818
GAGTACT50500.022.20318812
TATGCCG2207.2759576E-1221.9424443
AGAGTAC71350.021.90420711
CAGAGTA74400.021.09899510
GCACCGT2800.021.0512456
GCAGAGT77050.020.8175939
AGTACTT54550.020.68122913
GTACTTT54500.020.38368614
Produced by FastQC (version 0.11.5)