FastQCFastQC Report
Mon 6 Feb 2017
SRR2078218_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078218_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1109852
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12450.035.1669431
GGTATCA6500.030.7620221
TCAACGC15150.026.846724
ACCGTCG658.7055494E-426.5130028
CAACGCA15300.026.3582325
ATCAACG15650.025.9889983
GGACCGT1608.0035534E-1125.845516
TATCAAC16650.025.048022
AACGCAG16250.025.0237566
CGTGCCG1701.6916601E-1024.33506810
GCGATAC1502.9094736E-822.9831214
ACCGTGC3600.022.9779368
CGCCCAA1554.0652594E-822.26886650
TACCGAT1554.1338353E-822.2337027
TCGCAAT1253.510262E-622.07475749
GTACATG26800.021.9969041
GTGCCGA1756.2536856E-921.669811
GCACCGT2600.021.2065756
TACATGG27950.020.7171382
TGCGATA1701.093249E-720.27922213
Produced by FastQC (version 0.11.5)