FastQCFastQC Report
Mon 6 Feb 2017
SRR2078218_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078218_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1109852
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21000.042.6152951
GGTATCA8300.038.4480971
TGCGATA1207.6397555E-1131.63854213
ATCAACG30250.029.2150083
GCGATAC1301.9645086E-1029.2048114
CAACGCA29950.029.1604985
TCAACGC30200.028.9191024
TATCAAC30550.028.7116342
AACGCAG31000.028.3963996
CGTGCCG1900.027.24850310
ACGCAGA36000.024.1788797
CTAGTAC1301.7679668E-723.9933223
CTCGTAT1452.0268999E-823.77969639
GTACATG26650.023.5584371
CGCAGAG37550.023.0628038
GTGCGAT1953.8198777E-1123.00984812
TAGGCCG953.069742E-421.78504623
CGTACTA1906.8030204E-1021.77326829
TACATGG28000.021.6684882
AGAGTAC38700.021.5828811
Produced by FastQC (version 0.11.5)