FastQCFastQC Report
Mon 6 Feb 2017
SRR2078217_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078217_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2349148
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15750.037.4431721
GTATCAA32150.032.6099171
TCAACGC39250.025.7390464
CAACGCA39850.025.437495
ATCAACG39950.025.2001343
AACGCAG41200.024.5940236
TATCAAC41850.024.3834972
TCAATCG2200.023.50844245
GTACATG60650.022.6312921
TACATGG63200.021.4375512
CGTATAC1304.953239E-621.2169723
ACGCAGA48650.020.7578667
ACATGGG64800.020.377953
CGCAGAG51600.019.3732178
CGACGCG900.00574216419.16319560
AGAGTAC49200.018.8533611
GAGTACT23950.017.7092912
TCGCAAT2952.910383E-1117.54817649
CATGGGG52300.017.5365624
CAGAGTA53950.017.32125710
Produced by FastQC (version 0.11.5)