FastQCFastQC Report
Mon 6 Feb 2017
SRR2078217_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078217_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2349148
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17100.040.759751
GTATCAA40600.039.032081
TATCAAC55100.028.1820162
ATCAACG56450.027.626713
TCAACGC56550.027.5778564
CGTGCCG3450.026.99975810
CAACGCA57350.026.9514435
AACGCAG59800.026.0211056
CGTATAC1451.9226718E-823.9008083
GTACATG60800.022.8132921
TACATGG62400.021.7744462
ACGCAGA72750.021.269187
CGCAGAG76150.020.1559648
GAGTACT48800.020.00699412
ACATGGG66400.019.5723883
AGAGTAC74000.019.44104211
GCACCGT5350.018.785596
AGTACTT52400.018.76414513
CAGAGTA78750.018.39983610
GTGCCGA4150.018.28899211
Produced by FastQC (version 0.11.5)