FastQCFastQC Report
Mon 6 Feb 2017
SRR2078216_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078216_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2291050
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27400.036.3745421
GGTATCA13550.033.3412781
TATCAAC36150.027.6583372
ATCAACG36900.026.9122473
CAACGCA37350.026.6809045
TCAACGC37300.026.4393354
AACGCAG39350.025.4007976
ACGCAGA46050.021.2555947
CGCAGAG48100.020.0665848
ACGGTTG3500.019.73725136
AGAGTAC44850.019.68379411
GTACATG57900.019.1790731
CGTGCCG4400.018.81004510
ATCCCGG6300.018.61872960
CGTGATC5950.018.55430456
GATCCCG6050.018.24682259
GTCTTAG6450.018.1789781
GTATATG6850.018.124341
CAGAGTA51050.018.03627210
GAGTACT22350.017.89829812
Produced by FastQC (version 0.11.5)