FastQCFastQC Report
Mon 6 Feb 2017
SRR2078216_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078216_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2291050
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39500.041.1598361
GGTATCA16500.038.0270421
TATCAAC56100.028.9685552
ATCAACG57800.028.112843
TCAACGC57800.027.9929564
CAACGCA58500.027.7172245
AACGCAG61300.026.3381446
ACGCAGA72450.022.1717517
CGACCGG953.077025E-421.7788627
CGCAGAG76350.020.9194438
GAGTACT49250.020.66257512
AGAGTAC76200.020.14529811
CTCGTAT2800.019.70511239
CGTGCCG3750.019.31775710
CAGAGTA82600.018.91850510
AGTACTT54400.018.76987813
TAATTCG1852.6606904E-718.6387559
GTACTTT55400.018.43107214
GTACATG56150.018.3977361
ACGTAAT1702.445775E-618.25580856
Produced by FastQC (version 0.11.5)