FastQCFastQC Report
Mon 6 Feb 2017
SRR2078215_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078215_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1842583
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17300.038.6670271
GTATCAA39100.035.8042371
ATCAACG49800.027.6176723
TCAACGC49800.027.4799844
CAACGCA50950.026.9259245
TTACGCG658.7098265E-426.5119177
AACGCAG53000.025.8809326
TATCAAC53600.025.851932
GTGGTAT8100.025.1160981
ACGCAGA61600.022.0444877
CGCAGAG62000.021.8466768
TGGTATC9200.020.9812772
GTACATG78000.020.9098871
AGAGTAC60750.020.77173211
TACATGG78800.020.5590652
TATCCGC1056.065615E-419.6945698
GAGTACT27500.019.68361712
ACATGGG79750.019.6663723
CAGAGTA66200.019.3741610
CGGCGTA1258.727889E-519.3248348
Produced by FastQC (version 0.11.5)